Journal: Journal of Proteome Research

Article Title: Combined Transcriptomic– 1 H NMR Metabonomic Study Reveals That Monoethylhexyl Phthalate Stimulates Adipogenesis and Glyceroneogenesis in Human Adipocytes

doi: 10.1021/pr200765v

Figure Lengend Snippet: Transcriptomic effects of MEHP on human adipocytes. Human preadipocytes ( n = 5) were differentiated in vitro into adipocytes for 11 days and then treated with MEHP (100 μM) or DMSO (0.1%, vehicle) for 24 h. (A) PCA analysis was conducted using expression data from the entire microarray (32 322 probesets). Black: DMSO, red: MEHP. (B, C) Gene ontology analyses were conducted on the 142 significantly upregulated and on the 85 downregulated transcripts, using DAVID (B) and KEGG (C) databases. P < 0.05 was used as cutoff for selecting significantly enriched biological pathways.

Article Snippet: Briefly, preadipocytes were grown in Promocell Preadipocyte Growth Medium containing 5% fetal calf serum, 1 μg/mL hydrocortisone, 10 ng/mL human recombinant growth factor, and 4 μL/mL heparin, until confluence (day 0).

Techniques: In Vitro, Expressing, Microarray

Journal: Journal of Proteome Research

Article Title: Combined Transcriptomic– 1 H NMR Metabonomic Study Reveals That Monoethylhexyl Phthalate Stimulates Adipogenesis and Glyceroneogenesis in Human Adipocytes

doi: 10.1021/pr200765v

Figure Lengend Snippet: Intracellular triglycerides in human adipocytes. Human preadipocytes ( n = 5) were differentiated in vitro into adipocytes for 11 days and then treated with MEHP (100 μM) or DMSO (0.1%, vehicle) for 24 or 48 h. Black: DMSO, red: MEHP. *: P < 0.05 compared to DMSO-treated cells, Wilcoxon signed-rank test.

Article Snippet: Briefly, preadipocytes were grown in Promocell Preadipocyte Growth Medium containing 5% fetal calf serum, 1 μg/mL hydrocortisone, 10 ng/mL human recombinant growth factor, and 4 μL/mL heparin, until confluence (day 0).

Techniques: In Vitro